| 基于多肽与金属离子作用的一种高选择性Cd2+荧光比率传感器 |
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| 引用本文： | 王召璐,冯慧云,李艳,许涛,薛泽春,李连之.基于多肽与金属离子作用的一种高选择性Cd2+荧光比率传感器[J].无机化学学报,2013,29(18). |
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| 作者姓名： | 王召璐 冯慧云 李艳 许涛 薛泽春 李连之 |
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| 作者单位： | 聊城大学化学化工学院, 聊城 252059,聊城大学化学化工学院, 聊城 252059,聊城大学化学化工学院, 聊城 252059,聊城大学化学化工学院, 聊城 252059,聊城大学化学化工学院, 聊城 252059,聊城大学化学化工学院, 聊城 252059 |
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| 基金项目： | 山东省自然科学基金(No.ZR2011BL002)资助项目。 |
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| 摘 要： | 利用固相多肽合成法合成了一种新的多肽,将该多肽与荧光基团Dansyl偶联制备了一种新的荧光化学比率传感器:Dansyl-Cys-Pro-Pro-Cys-Trp-NH2。利用荧光光谱研究了它与金属离子的相互作用。结果表明,与其它13种金属离子相比该多肽对金属Cd2+有很好的选择性。它能特异性识别Cd2+,具有水溶性好,响应时间快等优点。该肽对Cd2+具有很强的键合作用,其结合常数为3.0×1011L2·mol-2,检出限为11.5nmol·L-1。
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| 关 键 词： | 多肽 Cd2+ 荧光化学传感器 荧光比率 荧光共振能量转移 |
A High Selective Fluorescent Ratio Sensor for Cd2+ Based on the Interaction of Peptide with Metal Ion |
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| WANG Zhao-Lu,FENG Hui-Yun,LI Yan,XU Tao,XUE Ze-Chun and LI Lian-Zhi.A High Selective Fluorescent Ratio Sensor for Cd2+ Based on the Interaction of Peptide with Metal Ion[J].Chinese Journal of Inorganic Chemistry,2013,29(18). |
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| Authors: | WANG Zhao-Lu FENG Hui-Yun LI Yan XU Tao XUE Ze-Chun and LI Lian-Zhi |
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| Institution: | School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, Shandong 252059, China,School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, Shandong 252059, China,School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, Shandong 252059, China,School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, Shandong 252059, China,School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, Shandong 252059, China and School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, Shandong 252059, China |
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| Abstract: | Anew fluorescent ratio chemosensor (Dansyl-Cys-Pro-Pro-Cys-Trp-NH2) for metal ions has been synthesized via Fmoc solid-phase peptide synthesis. The interactions of the peptide with metal ions were investigated by fluorescence spectroscopy. Results showed that it showed high selectivity toward Cd2+ over other commonly coexistent metal ions. The peptide has the advantages of specific recognition to Cd2+, a good water-soluble and a fast response. The peptide tightly binds to Cd2+ with the binding constant of 3.0×1011 L2·mol-2 and the detection limit of 11.5 nmol·L-1. |
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| Keywords: | peptide Cd2+ fluorescent chemosensor fluorescent ratio fluorescence resonance energy transfer |
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