HPLC quantification of flavonoids and biflavonoids inCupressaceae leaves |
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| Authors: | A Romani C Galardi P Pinelli N Mulinacci D Heimler |
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| Institution: | (1) Dipartimento di Scienze Farmaceutiche, Università degli Studi di Firenze, Via G. Capponi 9, 50121 Firenze, Italy;(2) Dipartimento di Scienza del Suolo e Nutrizione della Pianta, Università degli Studi di Firenze, P. le delle Cascine 18, 50144 Firenze, Italy |
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| Abstract: | Summary The aim of this investigation was to obtain qualitative and quantitative profiles of the flavonoid and biflavonoid composition
of six cypress species—Cupressus funebris L.,Cupressus sempervirens L.,Cupressus glabra L.,Cupressus arizonica L.,Cupressus goveniana L., andCupressus lusitanica L. HPLC-diode-array detection (DAD), HPLC-MS, and HPTLC were used to identify the individual compounds. A chromatographic
method was optimized for identification and quantification of the main flavonoid glycosides and biflavonoids. The flavonoids
identified and calibrated were: rutin, quercetin glucoside, quercetin rhamnoside, and kaempferol 3-O-rhamnoside. The biflavonoids identified and calibrated were: cupressuflavone, amentoflavone, robustaflavone, hinokiflavone,
methylrobustaflavone, methylamentoflavone, and dimethylcupressuflavone. |
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| Keywords: | Column liquid chromatography-mass spectrometry LC-diode array detection Flavonols Biapigenin Cupressaceae leaves |
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