Photoregulation of DMA Photolyases in Broom Sorghum Seedlings |
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| Authors: | Megumi Hada,Gü nther Buchholz,Tohru Hashimoto,Osamu Nikaido,Eckard Wellmann |
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| Affiliation: | Department of Biology, Faculty of Science, Kobe University, Kobe, Japan;Institute of Biology II, Freiburg University, Freiburg, Germany;Department of Life Science, Kobe Women's University, Kobe, Japan;Division of Radiation Biology, Faculty of Pharmaceutical Sciences, Kanazawa University, Kanazawa, Japan |
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| Abstract: | ![]()
Abstract— Using dark-grown 3 day-old Sorghum bicolor seedlings, photorepair of cyclobutane pyrimidine dimers (CPD) by CPD-photoIyase and pyrimidine-(6–4)pyrimidinone photoproducts ([6–4]PP) by [6–4]PP-photolyase was studied in vivo and in vitro. The two types of DNA photoproducts were measured with specific monoclonal antibodies. Sorghum seedlings have different photorepair enzymes for CPD and [6–4]PP. The CPD-photolyase was found to be increased in response to light with maximal efficiency in the UVA/blue spectral range, whereas [6–4]PP-photolyase was not influenced by light. |
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