Flow-Injection Chemiluminescence as a Means of Studying the Metabolism of Unbound Chromium(III) in Rabbit Blood with On-Line Microdialysis Sampling

In this paper, we present an in-vivo, on-line, real-time analytical system for monitoring the metabolism of free chromium(III) in rabbit blood. This system includes microdialysis sampling, ion-exchange on-line separation and chemiluminescence detection. The results show that none of the co-existing substances in the blood, including protein and other small molecules, interfere with the determination. CrCl₃·6H₂O was administrated orally (0.5 g), and the microdialysis probe was utilized to sample rabbit blood with a perfusion rate of 10 µL min⁻¹. The dialytic efficiency of chromium(III) under the experimental conditions was 18.1 ± 5.1% (n=3). The concentration-time curve of chromium(III) is in accordance with the one-compartmental open model, the T_1/2 is 16.62 min.