Reversed-phase liquid chromatography of the twenty-two tetrachlorodibenzo-p-dioxin isomers on pyrenylethyl- and octadecylsilylated silica gel columns |
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Authors: | E R Barnhart D G Patterson N Tanaka M Araki |
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Institution: | Division of Environmental Health Laboratory Sciences, Centers for Disease Control, Atlanta, GA 30333. |
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Abstract: | To purify the tetrachlorobenzo-p-dioxins synthesized for chemical and biological reference standards, reversed-phase liquid chromatography on pyrenylethyl- and octadecylsilylated stationary phase (PYE and C18) columns was employed with 100% methanol. The pyrenylethyl phase satisfactorily separated isomers resulting from mixtures of reaction products which had not been adequately separated with conventional C18 or silica gel. The use of a single chromatographic mode, liquid chromatography, and a single mobile phase with columns of distinctly different properties, C18 and PYE, separates 20 of the 22 isomers from each other and from the 1246/1249 pair. (The observed properties of the two columns afford insight into steric and polarizability differences among isomers.) |
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