Signal amplification by substrate recycling on polyaniline/lactate oxidase/lactate dehydrogenase bienzyme electrodes |
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| Authors: | Asha Chaubey Krishan K Pande Manoj K Pandey Vijai S Singh |
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| Institution: | (1) Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K. S. Krishnan Marg, 110012 New Delhi, India;(2) Department of Biochemistry, L.L.R.M. Medical College, 250004 Meerut, India |
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| Abstract: | The bienzyme electrodes were fabricated by coimmobilization of lactate oxidase (LOD) and lactate dehydrogenase (LDH) onto
electrochemically prepared polyaniline (PANI) films. These PANI/LOD/LDH bienzyme electrodes were shown to provide signal amplification
by substrate recycling, making it possible to detect l-lactate at lower concentrations (0.1-1 mM). The PANI/LOD/LDH bienzyme electrodes were found to be stable for about 21 d at
4–10°C. |
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| Keywords: | Lactate oxidase lactate dehydrogenase substrate recycling coimmobilization bienzyme electrode |
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