Hybridization of DNA to bead-immobilized probes confined within a microfluidic channel |
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| Authors: | Kim Joohoon Heo Jinseok Crooks Richard M |
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| Institution: | Department of Chemistry and Biochemistry, The University of Texas at Austin, 1 University Station, A5300, Austin, Texas 78712-0165, USA. |
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| Abstract: | We report the factors influencing the capture of DNA by DNA-modified microbeads confined within a microfluidic channel. Quantitative correlation of target capture efficiency to probe surface concentration, solution flow rate, and target concentration are discussed. The results indicate that the microfluidic system exhibits a limit of detection of approximately 10(-10) M (approximately 10(-16) mol) DNA and a selectivity factor of approximately 8 x 10(3). Typical hybridization times are on the order of minutes. |
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