| 高效液相色谱法测定萨拉沙星 |
| |
| 引用本文： | 何娟,常使标,刘澎,郭瑞云,林素凤,邝爱燕.高效液相色谱法测定萨拉沙星[J].色谱,1999,17(4):393-394. |
| |
| 作者姓名： | 何娟 常使标 刘澎 郭瑞云 林素凤 邝爱燕 |
| |
| 作者单位： | 河南省分析测试中心!郑州，450002，河南省分析测试中心!郑州，450002，河南省分析测试中心!郑州，450002，河南省分析测试中心!郑州，450002，河南省分析测试中心!郑州，450002，河南省分析测试中心!郑州，450002 |
| |
| 摘 要： | 采用高效液相色谱法测定了萨拉沙星。色谱柱为μ－BondapakTMC18柱（3．9mm×300mm），流动相为V（乙腈）：V（甲醇）：V（2mmol／L磷酸，用三乙胺调pH3．5）＝30：5：65，用二极管阵列检测器检测，检测波长278nm，得到了满意的分离效果。
|
| 关 键 词： | 高效液相色谱法 萨拉沙星 紫外检测 |
Determination of Sarafloxacin by High Performance Liquid Chromatography (HPLC) |
| |
| He Juan, Chang Junbiao, Liu Peng, Guo Ruiyun, Lin Sufeng and Kuang Aiyan.Determination of Sarafloxacin by High Performance Liquid Chromatography (HPLC)[J].Chinese Journal of Chromatography,1999,17(4):393-394. |
| |
| Authors: | He Juan Chang Junbiao Liu Peng Guo Ruiyun Lin Sufeng and Kuang Aiyan |
| |
| Institution: | Henan Centre of Analysis and Test, Zhengzhou, 450002. |
| |
| Abstract: | A method for the separation and determination of Sarafloxacin by HPLC was developed. The sample was dissolved in a mixture of V(acetonitrile):V(water) = 1:1. Operating conditions were as follows: mu-Bondapak C18 column (3.9 mm x 300 mm), V(acetonitrile):V(methanol):V(2 mmol/L H3PO4, adjust pH 3.5 with triethylamine) = 30:5:65 as mobile phase with a flow rate of 1 mL/min, UV detection at 278 nm, and column temperature was 15 degrees C. Under the above conditions, Sarafloxacin and other impurities were separated from each other. The method was simple, rapid, sensitive and accurate. |
| |
| Keywords: | high performance liquid chromatography Sarafloxacin UV detection |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《色谱》浏览原始摘要信息 |
| 点击此处可从《色谱》下载免费的PDF全文 |
|
