| 高效液相色谱多波长定性方法研究 |
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| 引用本文： | 陈笑梅,郑自强,施旭霞.高效液相色谱多波长定性方法研究[J].色谱,1999,17(2):178-181. |
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| 作者姓名： | 陈笑梅 郑自强 施旭霞 |
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| 作者单位： | 浙江进出口商品检验局!杭州，310012，浙江进出口商品检验局!杭州，310012，浙江进出口商品检验局!杭州，310012 |
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| 摘 要： | 介绍了高效液相色谱法中除常用的保留时间和光谱图定性以外的另一方法──多波长定性分析方法。根据Beer's定律可知，同一化合物在不同波长下测得的峰面积之比为定值。利用高效波相色谱多波长同时检测的方法，比较化合物在不同波长下的峰面积大小，建立了简便的定性方法，并在禁用假氮染料测定时对18种芳香腔的定性分析中得到成功的应用。
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| 关 键 词： | 定性分析 多波长检测 高效液相色谱法 |
Study on Qualitative Analysis Method of High Performance Liquid Chromatography by Simultaneous Multi-Wavelength Detection |
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| Chen Xiaomei, Zheng Ziqiang and Shi Xuxia.Study on Qualitative Analysis Method of High Performance Liquid Chromatography by Simultaneous Multi-Wavelength Detection[J].Chinese Journal of Chromatography,1999,17(2):178-181. |
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| Authors: | Chen Xiaomei Zheng Ziqiang and Shi Xuxia |
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| Institution: | Zhejiang Imp & Exp Commodity Inspection Bureau, Hangzhou, 310012. |
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| Abstract: | In this paper, we introduce an HPLC qualitative method , that is, multiwavelength detection qualitative analysis method. According to Beer's law, for a certain compounds the ratio of peak areas at different wavelengths is a constant. Comparing the ratio of peak areas of a component at different wavelengths with that of the standard by means of simultaneous multi-wavelength detection, we established a convenient qualitative method. This method has been applied successfully to qualitative analysis of 18 aromatic ammes in the prohibited azo dyes. |
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| Keywords: | high performance liquid chromatography multi-wavelength detection qualitative analysis |
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