Measurement of bubble size distribution in protein foam fractionation column using capillary probe with photoelectric sensors |
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Authors: | Du Liping Ding Yuqing Prokop Aleš Tanner Robert D |
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Institution: | (1) Chemical Engineering Department, Vanderbilt University, 37235 Nashville, TN |
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Abstract: | Bubble size is a key variable for predicting the ability to separate and concentrate proteins in a foam fraction ation process.
It is used to characterize not only the bubble-specific interfacial a rea but also coalescence of bubbles in the foam phase.
This article describes the development of a photoelectric method for measuring the bubble size distribution in both bubble
and foam columns for concentrating proteins. The method uses a vacuum to withdraw a stream of gas-liquid dispersion from the
bubble or foam column through a capillary tube with a funnel-shaped inlet. The resulting sample bubble cylinders are detected,
and their lengths are calculated by using two pairs of infrared photoelectric sensors that are connected with a high-speed
data acquisition system controlled by a microcomputer. The bubble size distributions in the bubble column 12 and 1 cm below
the interface and in the foam phase 1 cm above the interface are obtained in a continuous foam fractionation process for concentrating
ovalbumin. The effects of certain operating conditions such as the feed protein concentration, superficial gas velocity, liquid
flow rate, and solution pH are investigated. The results may prove to be helpful in understanding the mechanisms controlling
the foam fractionation of proteins. |
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Keywords: | Bubble size capillary tube photoelectric method foam fractionation ovalbumin |
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