| A cDNA CLONE WITH ANTIONCOGENE ACTIVITY |
| |
| 作者姓名： | 刘定干 王达 陈珍珍 李载平 野田亮 井川洋二 |
| |
| 作者单位： | Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC，Tsukuba Life Science Center，Institute of Physical and Chemical Research，Japan，Tsukuba Life Science Center，Institute of Physical and Chemical Research，Japan |
| |
| 基金项目： | This work was supported by a grant from the 863 Program, the National Commission on Science and Technology, China. |
| |
| 摘 要： | A cDNA clone, p14--6, which has an antioncogene activity on the v--Ki--Ras oncogene-transformed malignant cell line DT, was found. This clone was recovered from the revertantR14 cells, which had been isolated by transfections of DT cells with a normal human fibro-blast cDNA library cloned in pcD2, an Okayama-Berg vector. When transfected into DT cells,p14--6 clone gave rise to phenotypical flat reversion in 5--15% of DT transfectant colo-nies. The p14--6--transfected flat cell line, RR, was proven to be a true revertant with signif-icantly reduced malignancy by in ritro and in riro malignancy tests. All other clones recov-ered from R14 cells were unable to cause this reversion. Molecular hybridizations showedthat the p14--6 was inserted into RR genome as tandem repeats, and no structural changewas found in the D--Ki--Ras oncogene in RR genome. These facts suggest that the antioncogeneactivity of the p14--6 clone on the DT cells may be exerted through expression of thecDNA contained in this clone. Possib
|
A cDNA CLONE WITH ANTIONCOGENE ACTIVITY |
| |
| LIU DING-GAN,WANG DA,CHEN ZHEN-ZHEN,LI ZAI-PING,MAKOTO NODA,YOJI IKAWO.A cDNA CLONE WITH ANTIONCOGENE ACTIVITY[J].Science in China(Chemistry),1992(7). |
| |
| Authors: | LIU DING-GAN WANG DA CHEN ZHEN-ZHEN LI ZAI-PING MAKOTO NODA YOJI IKAWO |
| |
| Institution: | LIU DING-GAN,WANG DA,CHEN ZHEN-ZHEN,LI ZAI-PING,MAKOTO NODA,YOJI IKAWO Shanghai Institute of Biochemistry,Academia Sinica,Shanghai 200031,PRC Tsukuba Life Science Center,Institute of Physical and Chemical Research,Japan |
| |
| Abstract: | A cDNA clone, p14--6, which has an antioncogene activity on the v--Ki--Ras oncogene-transformed malignant cell line DT, was found. This clone was recovered from the revertantR14 cells, which had been isolated by transfections of DT cells with a normal human fibro-blast cDNA library cloned in pcD2, an Okayama-Berg vector. When transfected into DT cells,p14--6 clone gave rise to phenotypical flat reversion in 5--15% of DT transfectant colo-nies. The p14--6--transfected flat cell line, RR, was proven to be a true revertant with signif-icantly reduced malignancy by in ritro and in riro malignancy tests. All other clones recov-ered from R14 cells were unable to cause this reversion. Molecular hybridizations showedthat the p14--6 was inserted into RR genome as tandem repeats, and no structural changewas found in the D--Ki--Ras oncogene in RR genome. These facts suggest that the antioncogeneactivity of the p14--6 clone on the DT cells may be exerted through expression of thecDNA contained in this clone. Possible reasons for the non-reversion of part of DT cells after p14--6 transfection isdiscussed. |
| |
| Keywords: | antioncogene cDNA transfection reversion |
| 本文献已被 CNKI 等数据库收录！ |
|
