Monolithic spin column extraction and GC-MS for the simultaneous assay of diquat,paraquat, and fenitrothion in human serum and urine |
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| Authors: | Takeshi Saito Tomokazu Fukushima Yuko Yui Shota Miyazaki Akihiro Nakamoto Akira Namera Sadaki Inokuchi |
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| Institution: | (1) Department of Emergency and Critical Care Medicine, Tokai University School of Medicine, Shimokasuya 143, Isehara Kanagawa, 259-1143, Japan;(2) GL Sciences Inc, Sayamagahara, Iruma Saitama, 358-0032, Japan;(3) Scientific Investigation Laboratory, Hiroshima Prefectural Police Headquarters, Kohnan 2-26-3, Naka-ku, Hiroshima 730-0825, Japan;(4) Department of Forensic Medicine, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8551, Japan |
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| Abstract: | We present a method based on monolitic spin column extraction and gas chromatography–mass spectrometry as an analytical method
for screening diquat (DQ), paraquat (PQ), and fenitrothion in serum and urine. This method is useful for clinical and forensic
toxicological analyses. Recovery of DQ, PQ, and fenitrothion from serum and urine, spiked at concentrations between 0.1, 2.5,
20, and 45 μg/ml, ranged from 51.3% to 106.1%. Relative standard deviation percentages were between 3.3% and 14.8%. Detection
and quantitation limits for serum and urine were 0.025 and 0.05 μg/ml, respectively, for DQ, 0.1 and 0.1 μg/ml, respectively,
for PQ, and 0.025 and 0.05 μg/ml, respectively, for fenitrothion. Therefore, these compounds can be detected and quantified
in the case of acute poisoning. |
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