| 用原位PCR方法检测培养细胞中的单拷贝SRY基因 |
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| 引用本文： | 马琦,徐耀先,杨建琪,刘汀,张锡元.用原位PCR方法检测培养细胞中的单拷贝SRY基因[J].武汉大学学报(理学版),1998(2). |
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| 作者姓名： | 马琦 徐耀先 杨建琪 刘汀 张锡元 |
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| 作者单位： | 武汉大学生命科学学院，湖北医科大学生物系 |
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| 摘 要： | 以原位ＰＣＲ方法在培养的ＨＩＣＭＡ细胞中进行了Ｙ染色体上单拷贝ＳＲＹ基因的原位扩增，并使用ＰＣＲ方法制备的地高辛标记探针原位检测了所扩增的片段．比较研究表明，原位ＰＣＲ法较之直接的原位杂交法，其灵敏度提高５倍以上
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| 关 键 词： | 原位聚合酶链反应，原位杂交，培养细胞，SRY基因 |
DETECTION OF SRY GENE IN CULTURED CELLS BY IN SITU POLYMERASE CHAIN REACTION |
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| Ma Qi ,Xu Yaoxian ,Yang Jianqi ,Liu Ting ,Zhang Xiyuan.DETECTION OF SRY GENE IN CULTURED CELLS BY IN SITU POLYMERASE CHAIN REACTION[J].JOurnal of Wuhan University:Natural Science Edition,1998(2). |
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| Authors: | Ma Qi Xu Yaoxian Yang Jianqi Liu Ting Zhang Xiyuan |
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| Institution: | Ma Qi 1),Xu Yaoxian 2),Yang Jianqi 1),Liu Ting 1),Zhang Xiyuan 1) |
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| Abstract: | The single copy SRY gene was amplified by in situ PCR in cultured HICMA cells and the amplified products were detected with digoxigenin labeled probes generated using the PCR. Comparative studies indicated that in situ PCR is more than 5 times sensitive than in situ hybridization. |
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| Keywords: | in situ Polymerase Chain Reaction in situ Hybridization cultured cells SRY gene |
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