Analysis of carbohydrates using different quaternized polystyrene-divinylbenzene particles and pulsed amperometric detection |
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Authors: | M Jochum R Bakry I Wartusch C W Huck H Engelhardt G K Bonn |
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Institution: | (1) Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innrain 52a, 6020 Innsbruck, Austria;(2) Instrumental and Environmental Analytical Chemistry, University of Saarland, Im Stadtwald, 66123 Saarbrücken, Germany |
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Abstract: | Summary This paper reports on the use of a polymer-based, strong anion-exchange stationary phase for rapid, selective and sensitive
analysis of physiological important mono-, di- and oligosaccharides by high-performance anion-exchange chromatography (HPAEC)
with pulsed amperometric detection (PAD) under alkaline conditions. The adsorbent was obtained by direct nitration of 3 and
5 μm, spherical non-porous highly cross-linked, styrene-divinylbenzene copolymer (PS-DVB) beads, followed by reduction of
superficially introduced nitro groups with nascent hydrogen and quaternization of the resultant amino groups with iodomethane.
Extended exposure to high pressure and strong alkaline conditions did not have any untoward effect on mechanical stability
and chromatographic performance. A comparison of the 3 and 5 μm beads showed, that the synthesized 3 μm highly cross-linked
PS-DVB particles are the preferred phases for the separation of monosaccharides and the 5 μm particles are preferable for
the separation of oligosaccharides. To demonstrate the suitability for the analysis of complex samples, the optimized and
validated system was used for the determination of glucose, fructose and sucrose in apple juice and other soft drinks such
as Coca Cola. Finally, analysis within a few minutes without sample pretreatment down to a lower limit of detection of 0.174–0.504
μg mL−1 at a linearity with R2>0.994 and a repooducibility higher than 98% further confirmed the efficiency of these polymeric sorbents. |
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Keywords: | Column liquid chromatography Anion exchange chromatography Polymeric bonded phase Pulsed amperometric detection Mono- di- oligosaccharides |
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