Rapid enrichment of phosphopeptides by BaTiO3 nanoparticles after microwave-assisted tryptic digest of phosphoproteins, and their identification by MALDI-MS期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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**Figure**
*BaTiO₃ NPs as affinity probes for the rapid analysis of phosphopeptides by MALDI MS*
Rapid enrichment of phosphopeptides by BaTiO3 nanoparticles after microwave-assisted tryptic digest of phosphoproteins, and their identification by MALDI-MS

Authors:	Suresh Kumar Kailasa Hui-Fen Wu

Institution:	1. Department of Chemistry, National Sun Yat Sen University, Kaohsiung, 804, Taiwan 2. Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan 3. Department of Applied Chemistry, S. V. National Institute of Technology, Surat, 395007, India 4. Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung, 80424, Taiwan

Abstract:	We show that BaTiO₃ nanoparticles (NPs) can be used as a novel substrate for the rapid enrichment of phosphopeptides from microwave tryptic digests of α-casein and non-fat milk prior to their identification by MALDI-MS. Protein digestion is achieved by microwave tryptic digest for 50?s, and the resulting phosphopeptides can be effectively adsorbed on the surfaces of the NPs. The phosphopeptides were selectively detected via MALDI-MS. Digestion, enrichment and detection are accomplished within ～60?min. The method was applied to the indentification of 24 phosphopeptides from α-casein and of 21 phosphopeptides (of the α-casein type) from nonfat milk.

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