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Determination of sulfamethoxazole in milk using molecularly imprinted polymer monolith microextraction coupled to HPLC |
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| Authors: | Na Sun Shengli Wu Huaixia Chen Daijun Zheng Junwen Xu Yong Ye |
| Institution: | 1. Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, People’s Republic of China |
| Abstract: | We report on a new method for the selective extraction of the antibiotic sulfamethoxazole (SMO) in milk that is making use of a molecularly imprinted polymer (MIP) monolith as the sorbent. The monolith was synthesized in the tip of a micropipette using SMO as the template and a combination of acrylamide and 4-vinylpyridine as the co-functional monomers. The monolith was connected to syringes in different sizes and used for microextraction without any other treatment and showed high selectivity and enrichment ability for SMO. It was applied to the selective extraction and sensitive determination of SMO in milk. The linear range is from 5–600?μg?L?1, the correlation coefficient (r2) is 0.9984, and the detection limit (at S/N?=?3) is 1?μg?L?1. Recoveries range from 93.6 to 101.7?%, with relative standard deviations of <6.1?%. A method for the selective extraction of sulfamethoxazole (SMO) in milk based on molecularly imprinted polymer (MIP) monolith as the sorbent was developed. The linear ranges were 5–600?μg/L for SMO in milk. High recoveries of 93.6?～?101.7?% from milk were obtained with relative standard deviations less than 6.1?%. |
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