An enzyme-immunoassay for the detection of the mycotoxin zearalenone by use of yolk antibodies |
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| Authors: | H. Pichler R. Krska A. Székács M. Grasserbauer |
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| Affiliation: | (1) Center for Analytical Chemistry, Institute for Agrobiotechnology (IFA-Tulln), Konrad Lorenz Strasse 20, A-3430 Tulln, Austria, AT;(2) Plant Protection Institute, Hungarian Academy of Sciences, POB 102, H-1525 Budapest, Hungary, HU |
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| Abstract: | ![]()
The development of an enzyme-immunoassay (ELISA) for the detection of the Fusarium mycotoxin zearalenone (ZON) is described. In contrast to the common antibody isolation from mammal serum, chicken were immunized in order to isolate specific antibodies from the egg yolk. Five weeks after the start of the immunization a titer of 1:76 000 resulted from three injections without any adverse effects for the animals. Utilizing the indirect competitive assay format ZON could be detected in a concentration range between 10 and 200 μg/L. Received: 3 January 1998 / Revised: 16 March 1998 / Accepted: 20 March 1998 |
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