稀土对牙孢菌的抑菌机理研究

某些稀土盐类及其配合物抑菌性强、抑菌谱广，尤其对生命力极强的芽孢菌有 着更为突出的抑菌效果。本文的研究目的是探索稀土盐类及其配合物对芽孢菌的抑 菌机理；采用生物和化学方法，对芽孢进行破碎及成分的分离，借助电镜细胞化学 技术、红外光谱法、荧光分析法、原子吸收光谱法等检测手段，从分子水平上确定 了稀土离子在芽孢中的结合位点，提出了稀土对芽孢菌的抑菌机理是稀土离子取代 了芽孢内2,6-吡啶二羧酸钙(DPA-Ca~(2+))中Ca~(2+)的结合位点，形成了2,6-吡啶 二羧酸与稀土离子的配合物。

Studies on Bacteriostatic Mechanism of Rare Earth Compounds on Bacillus

Rare earth salts and rare earth complexes have strong and broad spectrum antibacterial effects, especially on Bacillus. This may correlate to the properties of the rare earth and the characteristics of Bacillus. In this paper, the spores of Bacillus are crashed and the small molecules in the spores are extracted with biological and chemical methods. By means of electronic microscopy, infrared spectrometry, fluorescence spectrometry and atomic absorption spectrometry, we have located the binding site of the rare earth ion in Bacillus at molecular level. Based on the above experiments, the bacteriostatic mechanism of the rare earth compounds is proposed. The rare earth bacteriostats not only destroy the special structure of the spores by the substitution of Ca 2+ in DPA-Ca 2+ with the rare earth ion, but also cause the loss of Ca 2+. Therefore, the bacteriostatic or bactericidal action occurs.