掺杂4-苯基卟啉的聚苯乙烯／二氧化硅复合纳米微球的制备

采用溶胶-凝胶法,首先利用苯乙烯与3-甲基丙烯酰氧基丙基三甲氧基硅烷(KH-570)化学反应合成共聚前驱物,利用TEOS在一定的条件下水解与缩合,一步合成了有机-无机复合纳米微球。用扫描电镜、红外光谱对共聚物及复合纳米粒子进行了表征。将非水溶性发光材料四苯基卟啉掺杂其中,制备出荧光复合纳米粒子。该粒子表现出了良好的发光性能,染料泄漏与猝灭几乎为零,可以作为一种新型的高效率的生物标记材料。

Preparation of Polystyrene/Silica Composite Nanoparticles Doped with Tetraphenylporphyrin

In a effort to prepare efficient and uniform biolabels,we describe a convenient, one-step sol-gel route to composite polymeric organic-inorganic nanosphere. First, the copolymer precursors were obtained via copolymerizations of styrene (St) and 3-methactyloxylpropyltrimethoxylsilane (KH-570), then the organic-inorganic hybrid materials have been prepared by properly controlling the condition of hydrolysis and condensation of sol-gel materials tetraethyl orthosilicate(TEOS). The copolymer precursors and their hybrid copolymers were explored and characterized by several methods including scanning electron microscope (SEM) and FTIR spectra. The scanning electron microscope(SEM)showed that the nanoparticles are very uniform, they are spherical and monodipersed with a diameter of about 200 nm. The FTIR spectra showed that the organic-inorganic nanoparticles contain polystyrene and silica two materials. The particles were also found to be good carriers for entrapped hydrophobic dyes such as phenylporphyrin molecules. The fluorophores are well protected from the environment when they were doped into the polystyrene network. Because of the hydrophobe polystyrene, water molecules can not contact the phenylporphyrin molecules inside.The outside hydrophilic silica network also help the nanoparticles disperse into the water. The method significantly reduces fluorescent dyes leaching and quenching from the nanoparticles. The emission maximum, compared with the phenylporphyrin DMF solution,change a little. It indicates that this is a physical method for doping dyes. The fluorescent nanoparticles prepared by using the novel method have the advantages such as high fluorescent and good fluorescent stability. The particles are potential of good biocompatibility, since they are a pure silica surfure and can thus be modified easily with many biomolecules for added biochemical functionality. The particles can be accepted as an effective biological labels.