Resolution of (D,L)-Phenylalanine in Biphasic System by α-Chymotrypsin Immobilized on Superparamagnetic Nanogels |
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| Authors: | Jiahui Yu Chengyun Wang Jun Hong Jin Huang |
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| Institution: | 1. College of New Drug Innovation Research &2. Development , East China Normal University , Shanghai, 200062, P. R. China;3. Development , East China Normal University , Shanghai, 200062, P. R. China;4. College of Chemical Engineering , Wuhan University of Technology , Wuhan, 430070, P. R. China |
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| Abstract: | α-Chymotrypsin immobilized on super paramagnetic nanogels covered with carboxyl groups, exhibited high stereoselectivity in kinetic resolution of racemic phenylalanine in toluene/water biphasic system, and even improved reusability over its parent free enzyme. The results also showed that the addition of ionic liquid to water phase could enhance the stereoselectivity of the immobilized enzyme to a certain extent while the conversion yield decreased. The long-term stability allows the supported enzyme with nano-scaled dimension to use in continuous chiral resolution reaction, which opens a new horizon for enzymatic chiral resolution in large-scale production. |
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| Keywords: | Superparamagnetic nanogels enzymic resolution immobilized α-chymotrypsinc (DL)-Phenylalanine ionic liquid |
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