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A new detection method for purines in thin-layer chromatography |
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| Authors: | C. Sârbu C. Măruţoiu |
| Affiliation: | (1) Faculty of Mechanical Engineering, Polytechnic Institute, 3400 Cluj-Napoca, România;(2) Chemical Institute, 3400 Cluj-Napoca, România |
| Abstract: | Summary The separation of some purines by thin-layer chromatography, utilizing precoated plates with Silicagel R as stationary phase and the following mobile phases: i-butanol: methylethylketone: ammonium hydroxide 25%: water (40  252015) and n-propanol: ammonium hydroxide 25%: water (80155) is reported.Visualisation of the spots has been achieved by spraying with aqueous 1% uranyl acetate and irradiating with UV both at 254 and 366 nm. The visual detection of a spot was 0.01  g. |
| Keywords: | Thin-layer chromatography Detection of purines Uranyl salts |
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