Improved quantitation of 13-cis- and all-trans-acitretin in human plasma by normal-phase high-performance liquid chromatography. |
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| Authors: | E Meyer W E Lambert A P De Leenheer J P Bersaques A H Kint |
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| Institution: | Laboratoria voor Medische Biochemie en voor Klinische Analyse, Faculteit Farmaceutische Wetenschappen, Rijksuniversiteit Gent, Belgium. |
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| Abstract: | A reliable analytical method has been developed for measurement of 13-cis- and all-trans-acitretin (Neotigason) in human plasma by normal-phase high-performance liquid chromatography, with ultraviolet detection. Human plasma was obtained after centrifugation of whole blood samples and deproteinized by ethanolic denaturation. After liquid-liquid extraction with water-n-hexane, and aliquot ws chromatographed on a silica column using isocratic elution with n-hexane-methylsalicylate-acetic acid (200:18:0.6, v/v). The wavelength was set at 360 nm, and for plasma samples a limit of quantification of 3-4 ng/ml was obtained. All manipulations were carried out under dim light conditions to prevent photoisomerization. |
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