Spin-trapped Radicals: Determination by LC-TSP-MS and LC-ESI-MS |
| |
Authors: | C E Parker H Iwahashi K B Tomer |
| |
Institution: | 1. Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, P.O. Box 12233, Z7709, Research Triangle Park, NC
|
| |
Abstract: | The 4-POBNα-(4-pyridyl-l-oxide)-N-tert-butyl-nitrone] radical adducts of ethyl and pentyl radicals were determined by a combination of high performance liquid chromatography (HPLC) combined with electron paramagnetic resonance (EPR) with HPLC-electrospray (ESI)-mass spectrometry and HPLC-thermospray (TSP)-MS. The identifIcation of the peak corresponding to the spin-trapped radical was done by performing HPLC-EPR under the same chromatographic conditions as the HPLC-MS. The radical adducts could be determined by both techniques, even though for ESI only 12 μL/min of the total 1 mL/min HPLC flow rate could be directed into the ion source. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|