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Dendrimer Functionalization with a Membrane‐Interacting Domain of Herpes Simplex Virus Type 1: Towards Intracellular Delivery |
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| Authors: | Tom P Carberry Rossella Tarallo Dr Annarita Falanga Dr Emiliana Finamore Prof Dr Massimiliano Galdiero Prof Dr Marcus Weck Prof Dr Stefania Galdiero |
| Institution: | 1. Molecular Design Institute and Department of Chemistry, New York University, New York, NY 10003 (USA), Fax: (+1)?212‐995‐4895;2. Dipartimeno di Scienze Biologiche, Università di Napoli “Federico II”, Via Mezzocannone 16, Napoli 80134 (Italy), Fax: (+39)?081‐253‐6642;3. Dipartimento di Medicina Sperimentale, Seconda Università degli Studi di Napoli, Via de Creccio 7, Napoli 80138 (Italy) |
| Abstract: | A poly(amide)‐based dendrimer was synthesized and functionalized with the membrane‐interacting peptide gH(625–644) (gH625) derived from the herpes simplex virus type 1 (HSV‐1) envelope glycoprotein H, which has previously been shown to assist in delivering large cargoes across the cellular membrane. We demonstrate that the attachment of the gH625 peptide sequence to the termini of a dendrimer allows the conjugate to penetrate into the cellular matrix, whereas the unfunctionalized dendrimer is excluded from translocation. The peptide‐functionalized dendrimer is rapidly taken into the cells mainly through a non‐active translocation mechanism. Our results suggest that the presented peptidodendrimeric scaffold may be a promising material for efficient drug delivery. |
| Keywords: | click chemistry delivery dendrimers membrane translocation peptides |