芘基丁酸键合硅胶固定相对含π电子物质的高效液相色谱分离研究

通过芘基丁酸键合硅胶固定相对含π电子物质的分离研究, 考察了该固定相的色谱性能. 结果表明, 该固定相不仅具有疏水作用和电荷转移性能, 还具有很强的平面识别能力, 可很好地分离烷基苯、 稠环芳烃及取代硝基苯等各种含π电子物质， 且在pH 3.5～7.0 时化学稳定性能良好, 硅醇基效应得到了很好的抑制, 可有效地用于碱性化合物的分离分析.

Separation of Solutes Containing π-Electron on Pyrenebutyric Acid-bonded Silica Stationary Phase by HPLC

The chromatographic performance of pyrenebutyric acid-bonded silica phase was evaluated by studying the separation of solutes containing π-electron. The results show that the PYB not only have the hydrophobicity and charge-transfer ability but also have the planar recognized ability, and could separate well such solutes containing  π-electron  as polycyclic aromatic hydrocarbon and some nitroaromatic hydrocarbons. The stationary phase has a high stability at a pH value between 3.5 and 7.0. The silanophilic activity of the PYB was weakened because of the existence of the amide group in the packing. It can be efficiently used for the analysis of basic compounds.