| 假丝酵母和白地霉的原生质体融合研究 |
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| 引用本文： | 段学辉,高鹤,颜淑芳,王志园.假丝酵母和白地霉的原生质体融合研究[J].南昌大学学报(理科版),2010,34(2). |
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| 作者姓名： | 段学辉 高鹤 颜淑芳 王志园 |
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| 作者单位： | 南昌大学,食品科学与技术国家重点实验室,江西,南昌,330047 |
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| 基金项目： | 江西省科技攻关基金资助项目(20041B0103000) |
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| 摘 要： | 对影响假丝酵母和白地霉原生质体制备与再生的因素:菌龄、破壁酶的种类和浓度、酶解时间、脱壁促进剂、渗透压稳定剂进行了研究。在综合比较原生质体制备率和再生率基础上,确定了制备原生质体的优化条件为:选用对数生长中期的菌体,用0.05%EDTA进行预处理,以0.7 mol/L NaCl为渗透压稳定剂,1.5%的纤维素酶30℃作用60 min。在此条件下,实验选育获得一株融合株CG16,其表型遗传性状和发酵性能稳定,产酶活力好,固态发酵脂肪水解酶活达到82.22 U/g干物质,比亲本假丝酵母和白地霉的产酶活力分别提高了32%和171%。
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| 关 键 词： | 脂肪酶 假丝酵母 白地霉 原生质体融合 |
Protoplast Fusion Between Candida valida T2 and Geotrichum Candidum NS3 |
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| DUAN Xue-hui,GAO He,YAN Shu-fang,WANG Zhi-yuan.Protoplast Fusion Between Candida valida T2 and Geotrichum Candidum NS3[J].Journal of Nanchang University(Natural Science),2010,34(2). |
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| Authors: | DUAN Xue-hui GAO He YAN Shu-fang WANG Zhi-yuan |
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| Institution: | State Key Laboratory of Food Science and Technology/a>;Nanchang University/a>;Nanchang 330047/a>;China |
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| Abstract: | The factors that affected the protoplasts formation and regeneration of Candida valida T2 and Geotrichum candidum NS3,such as culture age,species and concentration of digestion enzymes,enzymatic action time,reagents of promoting cell wall degradation and osmotic stability were studied.Through the comprehensive comparison experiment to the rates of formation and regeneration of protoplasts,results showed that the optimum preparation conditions for protoplasts of Candida valida T2 and Geotrichum candidum NS3 ... |
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| Keywords: | lipase candida valida geotrichum candidum protoplast fusion |
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