A new dark quencher for use in genetic analysis |
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| Authors: | May Jonathan P Brown Lynda J Rudloff Ivo Brown Tom |
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| Institution: | Department of Chemistry, University of Southampton, Highfield, Southampton, UK SO17 1BJ. |
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| Abstract: | A novel, long-wavelength, non-fluorescent quencher (LQ), based on 1,4-diaminoanthraquinone, has been incorporated at the 3' and 5'-termini of oligonucleotides. The quencher has been used in Molecular beacons, efficiently quenching the long wavelength fluorophore, Cy5. |
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