Efficient method for large-scale preparation of two components H and I of Sg-6 saponins from whole seeds of wild soybean (Glycine soja Sieb. and Zucc.) |
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| Authors: | Mei Itabashi Chigen Tsukamoto Ayaka Kurosaka Panneerselvam Krishnamurthy Tai-Sun Shin Seung Hwan Yang |
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| Institution: | 1. Department of Biomedical and Electronic Engineering, Chonnam National University, Yeosu, Republic of Korea;2. Faculty of Agriculture, Iwate University, Morioka, Japan;3. Faculty of Agriculture, Iwate University, Morioka, Japan;4. Soybean Applied Genomics Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, Japan;5. Division of Food and Nutrition, Chonnam National University, Gwangju, Republic of Korea;6. Department of Biotechnology, Chonnam National University, Yeosu, Republic of Korea |
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| Abstract: | New saponin components, Sg-6 saponins, have been recently reported from the seeds of wild soybean (Glycine soja) which may have specific health benefits. To evaluate the possible health benefits, a large amount of Sg-6 saponins are needed, but general group A acetyl saponins and new Sg-6 saponins are eluted in overlapping peaks by ordinal preparative high-performance liquid chromatography and/or open column methods. A new method is proposed in this report. This method includes (1) deacetylation of group A acetyl saponins in alkali condition with KOH, (2) precipitation of Sg-6 saponins in acid condition with HCl, (3) recovery of Sg-6 saponins with aqueous methanol from the precipitate, and (4) elution of Sg-6 saponins by preparative reverse-phase open column. With this method, from 450?g of wild soybean whole seed powder, about 1?g of Sg-6 saponins (mixture of six components) was clearly separated from other saponins with 61% recovery. |
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| Keywords: | Purification saponins separation soybean triterpene glycosides wild soybean (Glycine soja) |
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