In vitro anti-tumor activity of the tanshinone IIA against SKOV3 cells |
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| Authors: | Ju Huang Hao Lin |
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| Institution: | 1. Department of Gynaecology, The First Affiliated Hospital of Shantou University Medical College, Shantou, P.R. China;2. Department of Urology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, P.R. China |
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| Abstract: | The aim of this study was to determine the anti-tumour activity of tanshinone IIA in SKOV3 cells. Results suggested that tanshinone IIA could significantly inhibit (IC50 value = 19.6 μM) the proliferation and induce apoptosis of SKOV3 cells as demonstrated by flow cytometry analysis. In addition, tanshinone IIA treatment induced G2/M phase cell cycle arrest in SKOV3 cells. The results of Western blotting indicated that tanshinone IIA can suppress the expression of anti-apoptotic protein Bcl-2, increase (0.28 vs. 0.62) the expression of pro-apoptotic protein Bax (0.83 vs. 0.24) in SKOV3 cells. It can be concluded that the tanshinone IIA may be a possible therapeutic candidate having cytotoxic and anti-tumour potential. |
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| Keywords: | Tanshinone IIA SKOV3 apoptosis cell |
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