Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases |
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| Authors: | Jana Balintová Medard Plucnara Pavlína Vidláková Dr Radek Pohl Dr Luděk Havran Prof Dr Miroslav Fojta Prof Dr Michal Hocek |
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| Institution: | 1. Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead Sciences and IOCB Research Center, Flemingovo nam. 2, 16610 Prague 6 (Czech Republic), Fax: (+420)?220‐183‐559;2. Institute of Biophysics, v.v.i. Academy of Sciences of the Czech Republic, Kralovopolska 135, 61265 Brno (Czech Republic), Fax: (+420)?541‐211‐293;3. Central European Institute of Technology, Masaryk University Kamenice 753/5, 62500 Brno (Czech Republic);4. Department of Organic Chemistry, Faculty of Science, Charles University in Prague, Hlavova 8, 12843 Prague 2 (Czech Republic) |
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| Abstract: | Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane‐modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. In combination with nitrophenyl and aminophenyl labels, we have successfully developed a three‐potential coding of DNA bases and have explored the relevant electrochemical potentials. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications. |
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| Keywords: | DNA polymerase electrochemistry nucleoside triphosphates sequencing voltammetry |
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