Quantum Chemical Study of the Enzymatic Repair of T(6‐4)C/C(6‐4)T UV‐Photolesions by DNA Photolyases |
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Authors: | Dr Shirin Faraji Lukas Wirz Prof Dr Andreas Dreuw |
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Institution: | Interdisciplinary Center for Scientific Computing, Ruprecht‐Karls University, Im Neuenheimer Feld 368, 69120 Heidelberg (Germany), Fax: (+49)?(0)6221?548868 |
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Abstract: | Several strategies have evolved to repair one of the abundant UV radiation‐induced damages caused to DNA, namely the mutagenic pyrimidine (6‐4) pyrimidone photolesions. DNA (6‐4)‐photolyases are enzymes repairing these lesions by a photoinitiated electron transfer. An important aspect of a possible repair mechanism is its generality and transferability to different (6‐4) lesions. Therefore, previously suggested mechanisms for the repair of the T(6‐4)T lesion are here transferred to the T(6‐4)C and C(6‐4)T lesions and investigated theoretically using quantum chemical methods. Despite the different functional groups of the pyrimidine bases involved, a general valid molecular mechanism was identified, in which the initial step is an electron transfer coupled to a proton transfer from the protonated HIS365 to the N3′ nitrogen of the 3′ pyrimidine, followed by an intramolecular OH/NH2 transfer in one concerted step, which does not require an oxetane/azetidine or isolated water/ammonia intermediate. |
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Keywords: | DNA repair photolyases DNA cleavage quantum chemical calculations electron transfer |
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