Continuous production of butanol from starch-based packing peanuts |
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Authors: | Thaddeus C Ezeji Marisa Groberg Nasib Qureshi Hans P Blaschek |
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Institution: | (1) Department of Food Science and Human Nutrition, University of Illinois, Biotechnology and Bioengineering Group, 1207 W. Gregory Drive, 61801 Urbana, IL |
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Abstract: | Acetone, butanol, ethanol (ABE, or solvents) were produced from starch-based packing peanuts in batch and continuous reactors.
In a batch reactor, 18.9 g/L of total ABE was produced from 80 g/L packing peanuts in 110 h of fermentation. The initial and
final starch concentrations were 69.6 and 11.1 g/L, respectively. In this fermentation, ABE yield and productivity of 0.32
and 0.17 g/(L·h) were obtained, respectively. Compared to the batch fermentation, continuous fermentation of 40 g/L of starch-based
packing peanuts in P2 medium resulted in a maximum solvent production of 8.4 g/L at a dilution rate of 0.033 h−1. This resulted in a productivity of 0.27 g/(L·h). However, the reactor was not stable and fermentation deteriorated with
time. Continuous fermentation of 35 g/L of starch solution resulted in a similar performance. These studies were performed
in a vertical column reactor using Clostridium beijerinckii BA101 and P2 medium. It is anticipated that prolonged exposure of culture to acrylamide, which is formed during boiling/autoclaving
of starch, affects the fermentation negatively. |
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Keywords: | Starch-based packing peanuts continuous fermentation butanol Clostridium beijerinckii BA101 reactor dilution rate |
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