| 巴西果仁致敏原的实时荧光PCR检测 |
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| 引用本文： | 蒋丹,刘淑燕,徐君怡,齐震玉,王秋艳,曹际娟. 巴西果仁致敏原的实时荧光PCR检测[J]. 分子科学学报, 2009, 25(4) |
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| 作者姓名： | 蒋丹 刘淑燕 徐君怡 齐震玉 王秋艳 曹际娟 |
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| 作者单位： | 辽宁省出入境检验检疫局,辽宁,大连,116015 |
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| 基金项目： | 国家质量监督检验检疫总局公益性行业计划项目 |
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| 摘 要： | 针对巴西果仁2Salbumin mRNA基因设计引物、探针,在实时荧光PCR仪上进行扩增、检测和结果分析.结果显示:该组探针和引物对巴西果仁有很强的特异性,除巴西果仁外,其余8种对照树果材料均未检测到荧光信号,巴西果仁成分检测灵敏度达到0.1%.该方法具有灵敏度高、快速、简便的特点,可用于巴西果仁致敏原成分的定量检测.
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| 关 键 词： | 巴西果仁 致敏原 实时荧光聚合酶链式反应 |
Detection of brazil nut allergen by real-time PCR |
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| JIANG Dan,LIU Shu-yan,XU Jun-yi,QI Zhen-yu,WANG Qiu-yan,CAO Ji-juan. Detection of brazil nut allergen by real-time PCR[J]. Journal of Molecular Science, 2009, 25(4) |
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| Authors: | JIANG Dan LIU Shu-yan XU Jun-yi QI Zhen-yu WANG Qiu-yan CAO Ji-juan |
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| Affiliation: | Liaoning Entry-Exit Inspection and Quarantine Bureau;Dalian 116015;China |
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| Abstract: | The 2S albumin mRNA gene was selected to design the specific primers and probe for the detection of brazil nut by real-time PCR.The results show that the primers and probe are specific.No fluorescenc signal was detected in the other 8 control materials but brazil nut.The sensitivity of the detecting method could be 0.1%.The detecting method for brazil nut is sensitive,fast,and simple.And it can be applied for the quantitative detection of brazil nut allergen. |
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| Keywords: | Brazil nut allergen real-time PCR |
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