ON THE TYROSINATE INVOLVEMENT IN THE SCHIFF BASE DEPROTONATION IN THE PROTON PUMP CYCLE OF BACTERIORHODOPSIN |
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| Authors: | JOSEPH M FUKUMOTO JANE H HANAMOTO M A EL-SAYED† |
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| Institution: | Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90024, USA |
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| Abstract: | Abstract— The ultraviolet transient absorption assigned to the tyrosinate species in bacteriorhodopsin is followed in time and as a function of pH. Both its rise time and titration curve closely resemble those observed for the production of the M412 intermediate. These results may support a recently proposed mechanism that couples tyrosinate production to the Schiff base deprotonation in the proton pump of bacteriorhodopsin. |
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