Preparative isolation and purification of psoralen and isopsoralen from Psoralea corylifolia by high-speed counter-current chromatography |
| |
Authors: | Liu Renmin Li Aifeng Sun Ailing Kong Lingyi |
| |
Institution: | College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, China. renminliu@lctu.edu.cn |
| |
Abstract: | Psoralen and isopsoralen were separated from Psoralea corylifolia by high-speed counter-current chromatography (HSCCC). A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (5:5:4.5:5.5, v/v) was used for HSCCC separation, and yielded, from 100 mg of crude extract, 39.6 mg of psoralen and 50.8 mg of isopsoralen each at over 99% purity as determined by high performance liquid chromatography (HPLC). The identification of psoralen and isopsoralen were performed with 1H NMR and 13C NMR. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|