Determination of 4-aminopyridine in serum by solid-phase extraction and high-performance liquid chromatography. |
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| Authors: | A van der Horst P N de Goede H A van Diemen C H Polman H J Martens |
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| Affiliation: | Department of Pharmacy, Free University Hospital, Amsterdam, Netherlands. |
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| Abstract: | ![]()
An assay for the determination of 4-aminopyridine in serum has been developed using 3,4-diaminopyridine as internal standard and reversed-phase high-performance liquid chromatography with detection at 244 nm. A mobile phase of acetonitrile-methanol-ethanol-1% ammonium carbonate (75:10:10:5) provided excellent separation of both compounds. Samples were extracted on solid-phase columns. The linearity, precision, recovery and the limit of detection were all sufficient for the routine use of this assay in clinical studies of patients treated with 4-aminopyridine. |
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