β-Glucosidase production by Trichoderma reesei |
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Authors: | Tamás Juhász Anita Egyházi Kati Réczey |
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Institution: | (1) Department of Agricultural Chemical Technology, Budapest University of Technology and Economics, Szent Gellért tér 4, 1521 Budapest, Hungary |
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Abstract: | The hydrolysis of cellulose to the water-soluble products cellobiose and glucose is achieved via synergistic action of cellulolytic
proteins. The three types of enzymes involved in this process are endoglucanases, cellobiohydrolases, and β-glucosidases.
One of the best fungal cellulase producers is Trichoderma reesei RUT C30. However, the amount of β-glucosidases secreted by this fungus is insufficient for effective cellulose conversion.
We investigated the production of cellulases and β-glucosidases in shake-flask cultures by applying three pH-controlling strategies:
(1) the pH of the production medium was adjusted to 5.8 after the addition of seed culture with no additional pH adjustment
performed, (2) the pH was adjusted to 6.0 daily, and (3) the pH was maintained at 6.0 by the addition of Tris-maleate buffer to the growth medium. Different carbon sources—Solka
Floc 200, glucose, lactose, and sorbitol—were added to standard Mandels nutrients. The lowest β-glucosidase activities were
obtained when no pH adjustment was done regardless of the carbon source employed. Somewhat higher levels of β-glucosidase
were measured in the culture filtrates when daily pH adjustment was carried out. The effect of buffering the culture medium
on β-glucosidase liberation was most prominent when a carbon source inducing the production of other cellulases was applied. |
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Keywords: | β -Glucosidase cellulase Trichoderma reesei RUT C30 Tris-maleate buffer endoglucanase activities |
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