Raman spectroscopic detection of physiology changes in plasmid-bearing <Emphasis Type="Italic">Escherichia coli</Emphasis> with and without antibiotic treatment |
| |
Authors: | Angela Walter Martin Reinicke Thomas Bocklitz Wilm Schumacher Petra Rösch Erika Kothe Jürgen Popp |
| |
Institution: | 1.Institut für Physikalische Chemie, Friedrich-Schiller-Universit?t,Jena,Germany;2.Institut für Mikrobiologie, Friedrich-Schiller-Universit?t,Jena,Germany;3.Institut für Photonische Technologien,Jena,Germany |
| |
Abstract: | Bacterial resistances against antibiotics are increasingly problematic for medical treatment of pathogenic bacteria, e.g.,
in hospitals. Resistances are, among other genes, often encoded on plasmids which can be transmitted between bacteria not
only within one species, but also between different species, genera, and families. The plasmid pDrive is transformed into
bacteria of the model strain Escherichia coli DH5α. Within this investigation, we applied micro-Raman spectroscopy with two different excitation wavelengths in combination
with support vector machine (SVM) and linear discriminant analysis (LDA) to differentiate between bacterial cultures according
to their cultural plasmid content. Recognition rates of about 92% and 90% are achieved by Raman excitation at 532 and 244 nm,
respectively. The SVM loadings reveal that the pDrive transformed bacterial cultures exhibit a higher DNA content compared
to the untransformed cultures. To elucidate the influence of the antibiotic, ampicillin-treated cultures are also comprised
within this study and are classified with rates of about 97% and 100% for 532 and 244 nm Raman excitation, respectively. The
Raman spectra recorded with 532 nm excitation wavelength show differences of the secondary protein structure and enhanced
stress-related respiration rates for the ampicillin-treated cultures. Independent cultural replicates of either ampicillin-challenged
or non-challenged cultures are successfully identified with identification rates of over 90%. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|