| Spectroscopic study on the interaction between[Hg（SCN）4]2- and hemoglobin |
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| 作者姓名： | Lun Fu Tian Zhong Fang Liu Ling Kong Xiao Li Hu Shao Pu Liu |
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| 作者单位： | Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China ; Institute of Mechanical Manufacturing Technology, Chinese Academy of Engineering Physics, Mianyang 621900, China |
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| 基金项目： | supported by the National Natural Science Foundation of China(No.20875078) |
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| 摘 要： | ![]()
The interaction between[Hg（SCN）4]2- and hemoglobin（Hb） under conditions that simulate a physiological environment was investigated by UV-vis spectroscopy,fluorescence spectroscopy,resonance Rayleigh scattering（RRS） spectroscopy and circular dichroism（CD） spectroscopy.The results obtained from the change of UV-vis and CD spectra,the quenching of Hb fluorescence and the enhancement of RRS intensity proved that a 10:1 type complex was formed between[Hg（SCN）4]2- and Hb.The possible mechanism suggested for the interaction was that ten Hg（SCN）4]2- anions entered the four subunits of a Hb molecule to react with some residues to form an adduct by coordination and electrostatic forces.The coordination of[Hg（SCN）4]2- with Trp was the major cause of the fluorescence quenching of Hb.
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| 关 键 词： | [Hg（SCN）4]2- Hemoglobin Toxicological evaluation Molecular spectroscopy |
| 收稿时间： | 2012-07-25 |
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