葡萄酒中白藜芦醇的固相萃取GC/MS法测定及其生理活性的初步研究

用反向C₁₈固相萃取小柱对葡萄酒进行了预处理,然后用BSTFA硅烷化试剂对萃取物进行了衍生化处理,用气相色谱/质谱对葡萄酒中顺式、反式白藜芦醇进行了同时测定,建立了定量分析方法.对方法的精密度和回收率进行了测定,方法的相对标准偏差分别为4.48%(顺式)和5.23%(反式).顺式、反式白藜芦醇的平均回收率分别为94.4%和97.6%.并对10种国产葡萄酒和两种进口葡萄酒样品进行了测定.对白藜芦醇的生理活性也进行了实验,初步探讨了白藜芦醇对大鼠血管平滑肌细胞(VSMC)、大肠癌细胞株Lovo细胞和小鼠成纤维株3T3细胞的生长生殖活性的影响,观察了其形态变化.

Quantitative Determination of Resveratrols in Wine by GC/MS and Elementary Study on Their Physiological Activity

C 18 SPE method was applied to the extraction of the resveratrols from Chinese wine. GC/MS has been used to analyze two isomers of resveratrol, cis and trans resveratrol in wine simultaneously. Identification was based on retention time and mass ion spectrum. These two compounds were quantitative determined. The precision of the method for the determination of cis and trans resveratrol was 4 48% and 5 23% of RSD, respectively. The recovery of cis and trans resveratrol was 94 4% and 97 6%, respectively. Twelve kinds of actual wine samples were determined. The elementary study was also performed for physiological activity of resveratrols. The effects of resveratrols on growth and reproductive activity of VSMC, LOVO, 3T3 cells of mouse were preliminary researched and the morphological change of these cells had been observed.