| 基于荧光探针噻唑橙与核酸适体构象变化的钾离子检测 |
| |
| 引用本文： | 高淑丽,徐慧,鞠婧,狄祥,赵小梅,柳全文.基于荧光探针噻唑橙与核酸适体构象变化的钾离子检测[J].分析测试学报,2011,30(2):115-120. |
| |
| 作者姓名： | 高淑丽 徐慧 鞠婧 狄祥 赵小梅 柳全文 |
| |
| 作者单位： | 鲁东大学,化学与材料科学学院,山东,烟台,264025 |
| |
| 基金项目： | 山东省中青年科学家科研奖励基金资助项目，鲁东大学创新团队建设项目，山东省高等学校科技计划项目 |
| |
| 摘 要： | 建立了一种基于核酸适体(Aptamer)构象效应和荧光探针噻唑橙(TO)为荧光分子开关进行钾离子检测的光学方法.室温下钾离子可与Aptamer结合形成G-四面体结构,使双链解链变为四面体结构和单链,从而导致TO荧光强度降低.考察了TO浓度、反应温度及反应时间的影响.在最佳实验条件下,钾离子浓度在1.0×10-6 ～2....
|
| 关 键 词： | 噻唑橙 钾离子 核酸适体 |
Detection of Potassium Ion Based on Conformational Change of Aptamer and Fluorescent Probe Thiazole Orange |
| |
| GAO Shu li,XU Hui,JU Jing,DI Xiang,ZHAO Xiao mei,LIU Quan wen.Detection of Potassium Ion Based on Conformational Change of Aptamer and Fluorescent Probe Thiazole Orange[J].Journal of Instrumental Analysis,2011,30(2):115-120. |
| |
| Authors: | GAO Shu li XU Hui JU Jing DI Xiang ZHAO Xiao mei LIU Quan wen |
| |
| Institution: | GAO Shu-li,XU Hui,JU Jing,DI Xiang,ZHAO Xiao-mei,LIU Quan-wen(School of Chemistry and Material Sciences,Ludong University,Yantai 264025,China) |
| |
| Abstract: | An optical method for the detection of potassium ion based on the conformational change of aptamer and fluorescent probe thiazole orange(TO)was reported.Anti-K+ aptamer was hybridized with its complementary strand which has a single base mutation and is shorter than aptamer.TO exhibited stronger fluorescence when it interacted with the double-strand DNA mentioned above.The fluorescence emission intensity of TO decreased when double-strand DNA was dehybridized due to the formation of G-quadruplex structure a... |
| |
| Keywords: | thiazole orange potassium ion aptamer |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《分析测试学报》浏览原始摘要信息 |
| 点击此处可从《分析测试学报》下载免费的PDF全文 |
|
