| 水溶性CdSe量子点的合成及其作为荧光探针对大肠杆菌的快速检测 |
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| 引用本文： | 蔡朝霞,阮晓娟,石宝琴,夏万奎,马美湖. 水溶性CdSe量子点的合成及其作为荧光探针对大肠杆菌的快速检测[J]. 分析试验室, 2011, 30(3): 107-110 |
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| 作者姓名： | 蔡朝霞 阮晓娟 石宝琴 夏万奎 马美湖 |
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| 作者单位： | 华中农业大学食品科学技术学院,武汉,430070 |
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| 基金项目： | 华中农业大学学科交叉研究项目 |
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| 摘 要： | 采用水相法以谷胱甘肽为稳定剂合成高稳定性的CdSe量子点,利用化学偶联剂的作用使得量子点表面基团与菌体之间的成功结合,对偶联的条件进行了优化.基于荧光分析法建立了一种快速简便的大肠杆菌检测定量分析方法.研究结果表明:合成的量子点具有稳定、荧光性能良好等突出优点.通过偶联剂量子点能与大肠杆菌结合,其荧光强度与大肠杆菌浓度...
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| 关 键 词： | 量子点 大肠杆菌 荧光探针 快速检测 |
The synthesis of water-soluble CdSe quantum dots as fluorescence probe for the quick detection of Escherichia coli |
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| CAI Zhao-xia,RUAN Xiao-juan,SHI Bao-qin,XIA Wan-kui,MA Mei-hu. The synthesis of water-soluble CdSe quantum dots as fluorescence probe for the quick detection of Escherichia coli[J]. Chinese Journal of Analysis Laboratory, 2011, 30(3): 107-110 |
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| Authors: | CAI Zhao-xia RUAN Xiao-juan SHI Bao-qin XIA Wan-kui MA Mei-hu |
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| Affiliation: | CAI Zhao-xia,RUAN Xiao-juan,SHI Bao-qin,XIA Wan-kui and MA Mei-hu(Food Science and Technology College,Huazhong Agriculture University,Wuhan 430070) |
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| Abstract: | Stable CdSe quantum dots were synthesized by an aqueous phase route and the optimum conditions were found.Escherichia coli(E.coli) was successfully coupled to the CdSe quantum dots by using chemical coup ling reagents,and the optimum coupling condition was found.A new fluorescence measurement method for detection of E.coli count by using water-soluble CdSe quantum dots was develped.The bacterial images were obtained using fluorescence microscopy.The results showed that CdSe quantum dots were highly stable,h... |
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| Keywords: | Quantum dots Escherichia coli Fluorescence probe Quick determination |
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