β-环糊精衍生化胰酶的合成及其手性分离性能研究

研究了β-环糊精衍生化胰酶的合成以及作为毛细管电色谱手性选择剂的分离性能. 利用乙二醇二环氧丙烷醚作为交联剂, 将β-环糊精接枝到胰酶蛋白的主链, 得到了β-环糊精衍生化胰酶. 将其通过化学键合连接到毛细管柱内壁, 制备了β-环糊精衍生化胰酶毛细管电色谱柱. 在加压毛细管电色谱模式下, 利用该柱分离了色氨酸、扑尔敏、布洛芬、异丙嗪和阿托品等对映异构体, 得到了理想的分离效果, 且在分离扑尔敏时, 随着电压的增加, 对映异构体分离的分离度和相对保留时间均增加.

Synthesis and Properties of β-Cyclodextrin Derivatized Pancreatin Used as Chiral Selector in CEC

The synthesis and properties of β-cyclodextrin derivatized pancreatin used as chiral selector were investigated in this paper. Such β-cyclodextrin derivatized pancreatin was synthesized using β-cyclodextrin and protein in the presence of ethylene glycol diglycidyl ether in boric acid buffer at pH value 8.7. Amino group was coated on the internal surface of the silica capillary by sol-gel technology with triethoxylmethylsiloxane and (3-aminopropyl)trimethoxysiloxane. Covalent binding of β-cyclodextrin derivatized pancreatin was performed by glutaraldehyde. Tryptophan, chlorpheniramine, ibuprofen, promethazine and atroopine were baseline separated by β-cyclodextrin derivatized pancreatin in pressure capillary electrochromatography. High resolution and large relative retention time were obtained at high operation voltage in the separation of chlorpheniramine.