Quantification of Polycyclic Aromatic Hydrocarbons in Avian Dried Blood Spots by Ultra-performance Liquid Chromatography with Simple Liquid Extraction and Phospholipid Solid-phase Extraction Preparation |
| |
Authors: | Anthony A Provatas Cory A King Steven L Kolakowski James D Stuart Christopher R Perkins |
| |
Institution: | 1. Center for Environmental Sciences and Engineering, University of Connecticut, Storrs, CT, USAanthony.provatas@uconn.edu;3. Center for Environmental Sciences and Engineering, University of Connecticut, Storrs, CT, USA;4. Department of Chemistry, University of Connecticut, Storrs, CT, USA |
| |
Abstract: | Here, a simple, reliable method for the quantification of the 16 EPA priority polycyclic aromatic hydrocarbons in dried blood spots is outlined using liquid extraction and phospholipid solid-phase sample cleanup coupled with analysis by ultra-performance liquid chromatography with ultraviolet–visible detection. Whole blood spotted on Whatman FTA cards was efficiently quantified by extraction into acidified methanol and passed through a phospholipid solid-phase extraction well plate before injection into a liquid chromatography under reverse-phase conditions. The analyte recoveries in quality control samples ranged from 63.4 to 104.1%, with relative standard deviations from 0.48 to 2.04%. These figures of merit are comparable with measurements in whole blood or serum using similar techniques. The method detection limits were from 45.0?ng·g?1 for benzog,h,i]perylene to 118.7?ng·g?1 for chrysene, with matrix spike recoveries from 64.3 to 99.4%, demonstrating acceptable sensitivity and low matrix interference. With a simple liquid extraction approach and short 16-min liquid chromatography, the dried blood spots were effectively and rapidly analyzed. |
| |
Keywords: | Dried blood spots ultra-performance liquid chromatography (UPLC) polycyclic aromatic hydrocarbon (PAH) |
|
|