Structural characterisation of a water-soluble polysaccharide from tissue-cultured Dendrobium huoshanense C.Z. Tang et S.J. Cheng |
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| Authors: | Hua-Yang Si Nai-Fu Chen Cheng Huang Jun Li Hui Wang |
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| Institution: | 1. College of Biotechnology and Pharmaceutical Engineering, West Anhui University, Lu’an City, China;2. West Anhui Biotechnology Research Center of Natural Medicine and Traditional Chinese Medicine, West Anhui University, Lu’an City, China;3. School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China;4. School of Pharmacy, Anhui Medical University, Hefei, China;5. College of Life Sciences, Anhui Normal University, Wuhu City, China;6. Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, Anhui Normal University, Wuhu City, China |
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| Abstract: | A water-soluble polysaccharide TC-DHPA4 with a molecular weight of 8.0 × 105 Da was isolated from tissue-cultured Dendrobium huoshanense by anion exchange and gel permeation chromatography. Monosaccharide analysis revealed that the homogeneous polysaccharide was made up of rhamnose, arabinose, mannose, glucose, galactose and glucuronic acid with a molar ratio of 1.28:1:1.67:4.71:10.43:1.42. The sugar residue sequence analysis based on the GC-MS files and NMR spectra indicated that the backbone of TC-DHPA4 consisted of the repeated units:→6)-β-Galp-(1→6)-β-Galp-(1→4)-β-GlcpA-(1→6)-β-Glcp-(1→6)-β-Glcp-(→. The sugar residue sequences β-Glcp-(1→)-α-Rhap-(1→3)-β-Galp-(1→, β-Glcp-(1→4)-α-Rhap-(1→3)-β-Galp-(1→, β-Galp-(1→6)-β-Manp-(1→3)-β-Galp-(1→, and α-l-Araf-(1→2)-β-Manp-(1→3)-β-Galp-(1→ were identified as the branches attached to the C-3 position of (1→6)-linked galactose in the backbone. |
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| Keywords: | Dendrobium huoshanense C Z Tang et S J Cheng tissue-cultured polysaccharide isolation purification |
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