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High production of β-glucosidase from a marine Aspergillus niger immobilized on towel gourd vegetable sponges
作者姓名:Dong-Sheng Xue  Jiang-Bo Wang  Shan-Jing Yao
作者单位:a Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China; b Ministry of Education of Fermentation Key Laboratory and Collaboration Innovation Centre of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China
摘    要:To produce β-glucosidase by consecutive batch fermentation, a marine Aspergillus niger was immobilized on a natural carrier, towel gourd vegetable sponges. The immobilized mycelia were 0.15 g/g carrier with the immobilized biomass percentage of over 95%. The immobilized mycelia possessed the long durability(22.5 days). The maximum production occurred 1.5 day earlier by the immobilized mycelia than by the free mycelia. β-Glucosidase production of five consecutive batches was over 110 U/m L. At high salinity,the activity and stability of β-glucosidase from the marine A. niger increased remarkable. Immobilizing the marine A. niger on the novel natural carrier achieved the efficient production of β-glucosidase.


High production of β-glucosidase from a marine Aspergillus niger immobilized on towel gourd vegetable sponges
Dong-Sheng Xue,Jiang-Bo Wang,Shan-Jing Yao.High production of β-glucosidase from a marine Aspergillus niger immobilized on towel gourd vegetable sponges[J].Chinese Chemical Letters,2015,26(8):1011-1015.
Authors:Dong-Sheng Xue  Jiang-Bo Wang  Shan-Jing Yao
Institution:a Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China; b Ministry of Education of Fermentation Key Laboratory and Collaboration Innovation Centre of Industrial Fermentation, Hubei University of Technology, Wuhan 430068, China
Abstract:To produce β-glucosidase by consecutive batch fermentation, amarine Aspergillus niger was immobilized on a natural carrier, towel gourd vegetable sponges. The immobilized mycelia were 0.15 g/g carrier with the immobilized biomass percentage of over 95%. The immobilized mycelia possessed the long durability (22.5 days). The maximum production occurred 1.5 day earlier by the immobilized mycelia than by the free mycelia. β-Glucosidase production of five consecutive batches was over 110 U/mL. At high salinity, the activity and stability of β-glucosidase from the marine A. niger increased remarkable. Immobilizing the marine A. niger on the novel natural carrier achieved the efficient production of β-glucosidase.
Keywords:Marine Aspergillus niger  β-Glucosidase  Towel gourd vegetable sponges  
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