Fast high performance liquid chromatography and ultraviolet-visible quantification of principal phenolic antioxidants in fresh rosemary |
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Authors: | Troncoso N Sierra H Carvajal L Delpiano P Günther G |
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Institution: | Fundación Chile, Area Agroindustria, Parque Rabat 6165 Vitacura, Santiago, Chile. |
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Abstract: | An improved HPLC method is reported for the determination of rosemary's principal phenolic antioxidants, rosmarinic and carnosic acids, providing a fast and simultaneous determination for both of them by using a solid phase column. The analysis was performed with fresh methanolic extractions of Rosmarinus officinalis. To quantify the amount of antioxidants in a fast and reproducible way by means of UV-vis absorption measurements, a spectrophotometric multi-wavelength calibration curve was constructed based on the antioxidant contents obtained with the recently developed HPLC method. This UV-vis methodology can be extended to the determination of other compounds and herbs if the restrictions mentioned in the text are respected. |
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Keywords: | Carnosic acid Rosmarinic acid Carnosol Phenolic antioxidants HPLC analysis |
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