| 真空、低能离子注入对人宫颈癌（HeLa）细胞中 p53及c-fos mRNA的表达影响 |
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| 引用本文： | 张凤秋,方华圣,押辉远,秦广雍.真空、低能离子注入对人宫颈癌（HeLa）细胞中 p53及c-fos mRNA的表达影响[J].原子核物理评论,2008,25(4):385-390. |
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| 作者姓名： | 张凤秋 方华圣 押辉远 秦广雍 |
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| 作者单位： | 郑州大学物理工程学院, 河南 郑州 450052 |
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| 基金项目： | 国家科技攻关计划
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| 摘 要： | 在具有高效护水功能石蜡油的保护下进行了人宫颈癌细胞(HeLa细胞)的低能离子注入及真空处理， 用荧光定量PCR的方法分别研究了真空和30 keV N+的不同注入剂量下， 细胞中p53基因和c-fos基因的mRNA表达变化。 结果表明, 上述两个基因的表达与真空及低能离子注入之间存在着剂量关系。
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| 关 键 词： | 真空 低能离子注入 HeLa细胞 基因表达 实时定量PCR |
| 收稿时间： | 1900-01-01 |
Effect of Vacuum and Low-energy Ion Implantation on p53 and c -fos mRNA Expression in HeLa Cells |
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| ZHANG Feng-qiu,FANG Hua-sheng,YA Hui-yuan,QIN Guang-yong.Effect of Vacuum and Low-energy Ion Implantation on p53 and c -fos mRNA Expression in HeLa Cells[J].Nuclear Physics Review,2008,25(4):385-390. |
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| Authors: | ZHANG Feng-qiu FANG Hua-sheng YA Hui-yuan QIN Guang-yong |
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| Institution: | School of Physical Science & Technology, Zhengzhou University, Zhengzhou 450052, China |
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| Abstract: | The relationship between gene expression and low energy ion implantation has been studied. Because vacuum is prerequisite in low energy ion implantation， mineral oil was used to protect cells from water evaporating. In this study， HeLa cells were implanted by low energy ions (30 keV N+) at different doses, and p53 gene and c fos gene were studied with real time quantitative PCR. The result shows that gene expression changed obviously when cells were acted as vacuum control to the sample implanted 5×1014 ions/cm2. |
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| Keywords: | vacuum low-energy ion HeLa cell gene expression real-time quantitative PCR |
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