丝蛋白纤维机理的模型——应力作用下丝蛋白构象的转变

本文用喇曼(Raman)光谱和X-射线衍射等手段研究了桑蚕吐丝过程中丝腺体不同部位的丝蛋白的构象变化。发现随应力增加,从前部丝腺开始,丝蛋白产生了β-折叠构象。通过将丝蛋白直接在拉力机上拉伸,用Raman光谱等手段检测,详细研究了在不同拉伸条件下丝蛋白构象的变化,在微观层次上揭示了丝蛋白在应力作用下变性的全过程,并提出了相应的转变模型。

A MODEL OF THE FIBRILLIZATION MECHANISM OF SILK FIBROIN THE CONFORMATIONAL TRANSITION OF FIBROIN BY STRETCHING

In this paper, the conformational transition of silk fibroin in different parts of silkgland was determinated by Raman spectroscopy and LAXS. It was discovered that the β-sheet conformation of fibroin came into being gradually when the stress was increased. By stretching the fresh fibroin directly with Instron, their conformational transitions were studied by Raman spectroscopy. The denaturing process of silk fibroin under stress was revealed on micro-cosmic levels, and a relevant model was made.