1.Department of Chemistry,Government College University,Faisalabad,Pakistan;2.Department of Chemistry,Government College Women University,Faisalabad,Pakistan;3.Department of Chemistry,City University of Hong Kong,Kowloon,China;4.Department of Botany,University of Balochistan,Quetta,Pakistan;5.HEJ Research Institute of Chemistry, International Centre for Chemical and Biological Sciences,University of Karachi,Karachi,Pakistan;6.Department of Environmental Sciences and Engineering,Government College University,Faisalabad,Pakistan;7.Department of Chemistry,University of Sargodha,Sargodha,Pakistan;8.ORIC, Lahore College for Women University,Lahore,Pakistan;9.Department of Crop Science, Faculty of Agriculture,Universiti Putra Malaysia,Serdang,Malaysia
Abstract:
This research work was executed to determine chemical composition, anti-oxidant and anti-microbial potential of the essential oils extracted from the leaves and stem of Daphne mucronata Royle. From leaves and stem oils fifty-one different constituents were identified through GC/MS examination. The antioxidant potential evaluated through DPPH free radical scavenging activity and %-inhibition of peroxidation in linoleic acid system. The stem’s essential oil showed the good antioxidant activity as compared to leaves essential oil. Results of Antimicrobial activity revealed that both stem and leaves oils showed strong activity against Candida albicans with large inhibition zone (22.2?±?0.01, 18.9?±?0.20 mm) and lowest MIC values (0.98?±?0.005, 2.44?±?0.002 mg/mL) respectively. Leaves essential was also active against Escherichia coli with inhibition zone of 8.88?±?0.01 mm and MIC values of 11.2?±?0.40 mg/mL. These results suggested that the plant’s essential oils would be a potential cradle for the natural product based antimicrobial as well as antioxidant agents.
